uniQure NV美国存托凭证招股说明书（2026年6月24日版）

4,945,055 Ordinary Shares We are offering 4,945,055 ordinary shares, nominal value €0.05 per share, pursuant to this prospectussupplement and the accompanying prospectus. Our ordinary shares are listed on The Nasdaq Global Select Market, or Nasdaq, under the symbol“QURE.” On June 23, 2026, the last reported sale price of our ordinary shares on Nasdaq was $49.04 perordinary share. Investing in our ordinary shares involves significant risks. See “Risk Factors” beginning on pageS-7of thisprospectus supplement as well as the documents incorporated by reference into this prospectus supplement andthe accompanying prospectus for a discussion of the factors you should consider before deciding to purchase our Neither the Securities and Exchange Commission nor any state securities commission has approved ordisapproved of these securities or passed upon the adequacy or accuracy of this prospectus supplement or theaccompanying prospectus. Any representation to the contrary is a criminal offense. (1)See “Underwriting” beginning on pageS-25of this prospectus supplement for additional informationregarding total underwriting compensation. Delivery of our ordinary shares is expected to be made on or about June 25, 2026. We have granted theunderwriters an option for a period of 30days to purchase up to an additional 741,758 ordinary shares at thepublic offering price less the underwriting discounts and commissions. Joint Bookrunning Managers Leerink PartnersStifel GuggenheimSecurities RBC Capital Markets Lead Manager H.C. Wainwright & Co. The date of this prospectus supplement is June 23, 2026. TABLE OF CONTENTS ABOUT THIS PROSPECTUS SUPPLEMENT This prospectus supplement and the accompanying prospectus relate to an offering of our ordinaryshares. Before buying any of the ordinary shares that we are offering, we urge you to carefully read thisprospectus supplement and the accompanying prospectus, together with the information incorporated byreference herein and therein, including as described under the headings “Where You Can Find More This document is in two parts. The first part is this prospectus supplement, which describes the terms ofthe offering of the securities offered hereby and also adds to, updates and changes the information containedin the accompanying prospectus and the documents incorporated by reference into this prospectussupplement and the accompanying prospectus. The second part is the accompanying prospectus, whichprovides more general information, some of which may not apply to this offering and some of which mayhave been supplemented or superseded by information in this prospectus supplement or documentsincorporated or deemed to be incorporated by reference into this prospectus supplement that we filed withthe Securities and Exchange Commission, or SEC, subsequent to the date of the prospectus. To the extent This prospectus supplement and the accompanying prospectus are part of a registration statement thatwe filed with the SEC, utilizing a “shelf” registration process as a “well-known seasoned issuer” as definedin Rule405 under the Securities Act of 1933, as amended, or the Securities Act. Under this shelf registrationprocess, we may offer from time to time various securities, of which this offering of our ordinary shares is apart. Such registration statement also includes exhibits that provide more detail on the matters discussed inthis prospectus supplement and the accompanying prospectus. You should read this prospectus supplement, We are responsible for the information contained in or incorporated by reference in this prospectussupplement and the accompanying prospectus and in any free writing prospectus we prepare or authorize.We have not, and the underwriters have not, authorized anyone to provide you with information, or to makeany representation, different from that which is contained, or incorporated by reference, in this prospectus You should assume that the information contained in this prospectus supplement is accurate as of thedate on the front cover of this prospectus supplement only and that any information we have incorporated byreference or included in the accompanying prospectus is accurate only as of the date given in the documentincorporated by reference or as of the date of the prospectus, as applicable, regardless of the time of We are not, and the underwriters are not, making an offer to sell our ordinary shares in any jurisdictionwhere the offer or sale of such securities is not permitted. For investors outside the United States: Neitherwe, nor the underwriters, have done anything that would permit this offering or possession or distribution ofthis prospectus supplement or the accompanying prospectus in any jurisdiction where action for that We further note that the representations, warranties and covenants made by us in any agreement that isfiled as an exhibit to any document that is incorporated by reference into this prospectus supplement or theaccompanying