This prospectus supplement modifies, amends and supplements certain information contained in the Prospectus of DestinyTech100 Inc. (the “Company”) dated December 22, 2023, as amended or supplemented (the “Prospectus”) and statement of additionalinformation, dated December 22, 2023, as amended or supplemented (the “Statement of Additional Information”). Capitalized terms You should carefully consider the "Risk Factors" section beginning on page 16 of the Prospectus. Net Asset Value In connection with our regular net asset value determination process, as provided in our valuation policies and procedures, ournet asset valueas of March 31, 2025, is $6.31 per share of our common stock. Portfolio The following table sets forth certain information as of March 31, 2025, for each portfolio company in which we have invested.Percentage of portfolio column is based on an approximate portfolio value of $69.58million as of March 31, 2025. Total (1) The Company has a direct investment in an SPV which has invested in an underlying portfolio company. If applicable, thenumber of units presented, are the units in the SPV owned by the Company, which represents the equivalent number of securities of (2) The Company has a direct investment in an SPV which has economic exposure to an underlying portfolio company. Thenumber of units presented, if applicable, are the units in the SPV owned by the Company, which represents the equivalent number of (3) During the year ended December 31, 2024, the SPV disposed of the underlying asset. As of March 31, 2025, the SPVdoes not hold any underlying assets. (4) The SPV has invested through one underlying SPV, resulting in the related economic exposure to the Company. (5) The SPV has invested through five underlying SPVs, resulting in the related economic exposure to the Company. Five ofthe underlying SPVs have one additional layer of SPVs, while one has two layers. (6) Investment is an SPV that holds multiple forward agreements that represent common shares of the indicated portfoliocompany. Forward contracts involve the future delivery of shares of a portfolio company upon such securities becoming freely