扩大全球流感监测和应对系统的呼吸道合胞病毒测序考虑

Respiratory syncytial virussequencingconsiderationsfor an expanded GlobalInfluenza Surveillance andResponse System Respiratory syncytial virus sequencing considerations for an expanded Global Influenza Surveillance and ResponseSystem ISBN978-92-4-011154-7(electronic version)ISBN 978-92-4-011155-4 (print version) © World Health Organization2025 Some rights reserved. This work is available under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0IGO licence(CCBY-NC-SA3.0IGO;https://creativecommons.org/licenses/by-nc-sa/3.0/igo). Under the terms of this licence, you may copy, redistribute and adapt the work for non-commercialpurposes, providedthe work is appropriately cited, as indicated below. In any use of this work, there shouldbe no suggestion that WHO endorses any specific organization, products or services. The use of the WHOlogo is not permitted. If you adapt the work, then you must license your work under the same or equivalentCreative Commons licence. If you create a translation of this work, you should add the following disclaimeralong with the suggested citation:“This translation was not created by the World Health Organization(WHO). WHO is not responsible for the content or accuracy of this translation. The original English editionshall be the binding and authentic edition”. Any mediation relating to disputes arising under the licence shall be conducted in accordance with themediation rules of the World Intellectual Property Organization(http://www.wipo.int/amc/en/mediation/rules/). Suggested citation.Respiratory syncytial virus sequencing considerations for an expanded GlobalInfluenza Surveillance and Response System.Geneva: World Health Organization;2025. Licence:CCBY-NC-SA3.0IGO. Cataloguing-in-Publication (CIP) data.CIP data are available athttps://iris.who.int/. Sales, rights and licensing.To purchase WHO publications, seehttps://www.who.int/publications/book-orders. To submit requests for commercial use and queries on rights and licensing, seehttps://www.who.int/copyright. Third-party materials.If you wish to reuse material from this work that is attributed to a third party, such astables, figures or images, it is your responsibility to determine whether permission is needed for that reuseand to obtain permission from the copyright holder. Therisk of claims resulting from infringement of anythird-party-owned component in the work rests solely with the user. General disclaimers.The designations employed and the presentation of the material in this publicationdo not imply the expression of any opinion whatsoever on the part of WHO concerning the legal status ofany country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers orboundaries. Dotted and dashed lines on maps represent approximate border lines for which there may notyet be full agreement. The mention of specific companies or of certain manufacturers’ products does not imply that they areendorsed or recommended by WHOin preference to others of a similar nature that are not mentioned.Errors and omissions excepted, the names of proprietary products are distinguished by initial capital letters. All reasonable precautions have been taken by WHOto verify the information contained in this publication.However, the published material is being distributed without warranty of any kind, either expressed orimplied. The responsibility for the interpretation and use of the material lies with the reader.In no eventshall WHObe liable for damages arising from its use. Contents AcknowledgementsivAbbreviationsvRationaleviBackground1GoalsandobjectivesofRSVglobalgenomic surveillance based on GISRS2SpecimenselectionandprocessingforRSV genomic surveillance3RSVspecimencollection,storage,transportandhandling: laboratory considerations5RSVtesting:laboratoryconsiderations6RSVsequencing:bioinformaticconsiderations9Qualitycontrolofsequencingoutputsand external quality assessment14RSVgenomicdatasharing,monitoringand analysis15References18Annex1:Samplesizecalculations21 Acknowledgements The World Health Organization (WHO) acknowledges the contributions of the WHOrespiratory syncytial virus (RSV)Reference LaboratoriesandotherexpertsthatcontributedtothedevelopmentofthisguidancedocumentonrespiratorysyncytialvirussequencingconsiderationsforanexpandedGlobalInfluenzaSurveillanceandResponseSystem.Specific acknowledgement is given to the contributions made by Thomas Williams(Child Life and Health, University of Edinburgh, Edinburgh, United Kingdom of Great Britain and NorthernIreland); Ian Barr, Clyde Dapat, Yi-Mo Deng, Xiaomin Dong, and Steven Edwards (Victorian InfectiousDiseases Reference Laboratory, Peter Doherty Institute, Melbourne, Australia);Stephanie Goya(University of Washington, Seattle, United States of America); Buhle Ntozini and Nicole Wolter (Centre forRespiratory Diseases and Meningitis, National Institute for Communicable Diseases, Johannesburg,South Africa); Tina Talts,Joanna Ellisand Ma