424B31d383753d424b3.htm424(B)(3) FiledPursuanttoRule424(b)(3)RegistrationStatementNo.333-266825 ProspectusSupplementNo.1 toProspectusdatedSeptember27,2022 2,083,334AmericanDepositaryShares NanoLabsLtd Representing4,166,668ClassAOrdinaryShares ThisProspectusSupplementNo.1supplementsandamendsourprospectusdatedSeptember27,2022(the“Prospectus”),relatingtothepublicofferingof2,083,334Americandepositaryshares(“ADSs”)representingClassAordinarysharesofNanoLabsLtd. ThisprospectussupplementisbeingfiledtosupplementandupdatecertaininformationcontainedonthecoveroftheProspectusandinthesectionsentitled“ProspectusSummary,”“UseofProceeds,”“Capitalization,”“Dilution,”“Management’sDiscussionandAnalysisofFinancialConditionandResultsofOperations”and“Underwriting”intheProspectus.ThisprospectussupplementshouldbereadinconjunctionwiththeProspectusandanyamendmentsorsupplementsthereto,whicharetobedeliveredwiththisprospectussupplement,andisqualifiedbyreferencetotheProspectus,excepttotheextentthattheinformationinthisprospectussupplementupdatesorsupersedestheinformationcontainedintheProspectus,includinganyamendmentsorsupplementsthereto. InvestingintheADSsinvolvesrisk.Pleasereadcarefullythesectionentitled“RiskFactors”beginningonpage17oftheProspectus. NeithertheSecuritiesandExchangeCommissionnoranystatesecuritiescommissionhasapprovedordisapprovedthesesecuritiesordeterminediftheProspectusorthisprospectussupplementistruthfulorcomplete.Anyrepresentationtothecontraryisacriminaloffense. ThedateofthisProspectusSupplementNo.1isSeptember29,2022. ThefollowinginformationsupplementsandupdatestheinformationcontainedintheProspectus.AllreferencestopagenumbersaretopagenumbersintheProspectus. (1)ThetableonthecoveroftheProspectusisherebysupersededandreplacedbythefollowing: PerADS Total Publicofferingprice US$2.40 US$5,000,002 Underwritingdiscountsandcommissions(1) US$0.17 US$350,000 Proceeds,beforeexpenses,tous US$2.23 US$4,650,002 (2)Thelasttwosentencesofthefirstparagraphonpage9oftheProspectusareherebysupersededandreplacedbythefollowing: WeestimatethatthenetproceedstousfromthisofferingwillbeapproximatelyUS$4.4million(afterdeductingestimatedunderwritingdiscountsandcommissionsandestimatedofferingexpensespayablebyus),ofwhichapproximatelyUS$2.8millionwillbetransferredtoourPRCsubsidiariesfor(1)researchanddevelopmentinitiativesformoreadvancedASICchips,smart-NICs,visioncomputingchipsandourMetaversecomputingnetworkplatform,Ipolloverse,(2)theestablishmentofourmanufacturingplantforproductassemblingandsupplychainoptimization,and(3)workingcapitalandothergeneralcorporateactivitieswithinChina.See“UseofProceeds”formoredetails. (3)Thefirstsentenceoppositethesubtitle“UseofProceeds”onpage12oftheProspectusisherebysupersededandreplacedbythefollowing: WeestimatethatwewillreceivenetproceedsofapproximatelyUS$4.4million(orUS$5.1millioniftheunderwritersexerciseinfulltheiroptiontopurchaseadditionalADSs)fromthisoffering,afterdeductingunderwritingdiscountsandcommissionsandestimatedofferingexpensespayablebyus. (4)Thefirstsentenceonpage70oftheProspectusisherebysupersededandreplacedbythefollowing: WeestimatethatwewillreceivenetproceedsfromthisofferingofapproximatelyUS$4.4million,orapproximatelyUS$5.1millioniftheunderwritersexerciseinfulltheiroptiontopurchaseadditionalADSs,afterdeductingunderwritingdiscountsandcommissionsandestimatedofferingexpensespayablebyus. (5)Thetableonpage73oftheProspectusisherebysupersededandreplacedbythefollowing: AsofJune30,2022 ActualProFormaProFormaasAdjusted RMB US$RMB US$ RMB US$ Non-currentliabilities Long-termloan ——6,991,483 1,041,732 6,991,483 1,041,732 Shareholders’equity Ordinaryshares($0.0001par value;500,000,000shares authorized;103,790,000shares issuedandoutstandingasofJune30,2022) 66,970 9,979 — — — — ClassAordinaryshares($0.0001 parvalue;noneissuedand outstandingonanactualbasis asofJune30,2022, 50,151,846sharesoutstanding onaproformabasisasof June30,2022and54,318,514 sharesoutstandingonaproformaasadjustedbasisasofJune30,2022) — — 32,452 4,836 35,248 5,252 ClassBordinaryshares($0.0001 parvalue;noneissuedandoutstandingonanactualbasisasofJune30,2022,57,178,154sharesoutstandingonaproformabasisasofJune30,2022and57,178,154sharesoutstandingonaproformaas —— 36,894 5,497 36,894 5,497 Additionalpaid-incapital 206,092,05330,707,759312,417,16646,550,223341,799,96750,928,266 Accumulateddeficit ) (112,286,174 ) (16,730,663 ) (112,286,174 ) (16,730,663 ) (112,286,174 ) (16,730,663 Accumulatedothercomprehensiveloss ) ) (168,435(1,130,435 ) ) (168,435(1,130,435 ) ) (1,130,435 (168,435 Totalshareholders’equity 92,742,41413,818,640199,069,90329,661,458228,455,50034,039,917 Totalcapitalization 92,742,41413,818,640206,061,38630,703,190235,446,98335,081,649 (6)Thefirstparagraphonpage75oftheProspectusisherebysupersededandreplacedbythefollowing: WithouttakingintoaccountanyotherchangesinnettangiblebookvalueafterJune30,2022,otherthantogiveeffectto:(1)there-designationof57,178,154sharesbeneficiallyownedbyMr.JianpingKongandMr.QifengSun,thefounde